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KMID : 0903520070500010072
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2007 Volume.50 No. 1 p.72 ~ p.76
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Isolation and Characterization of Major Glycosphingolipid from Rice Bran Extract
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Kang Byoung-Won
Susumu Mitsutake
Takashi Okada
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Abstract
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In order to examine the biofunctions of glycosylceramide which is representative of sphingolipid, monoglycosylceramide (cerebroside) was isolated from rice bran extract. Crude glycosylceramides were isolated in large quantities and promptly by flash system column chromatography from rice bran extract, and purified by normal-phase HPLC using an evaporative light-scattering detector. One major cerebroside was obtained by HPLC used as an eluent consisting of chloroform, methanol and water (99 : 11 : 1, v/v/v), and the constituents were analyzed by MALDI/TOF-MS, FAB-MS, GC and 600 MHz 1H-NMR. The component sugar was estimated to be glucose. In the ceramide, the fatty acid component consist was 2-hydroxy arachidic acid. The long-chain base component was sphinga-4,8-dienine.
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KEYWORD
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sphingolipid, ceramide, edible plant, biofunction, cerebroside, rice bran extract
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